东华大学学报(英文版)

2019, v.36(05) 479-482

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Cloning and Expression of Extremely Heat-Resistant Endo-β-1,4-Glucanase Gene from Thermotoga maritima in Bacillus subtilis
Cloning and Expression of Extremely Heat-Resistant Endo-β-1,4-Glucanase Gene from Thermotoga maritima in Bacillus subtilis

郝亚荣;陈婷;张兴群;

摘要(Abstract):

Gene encoding endo-β-1,4-glucanase( TM1525) is derived from Thermotoga maritima( T. maritima),which has an open reading frame of 825 bp and encodes a 274 amino acid endo-β-1,4-glucanase. This enzyme has the same high temperature resistance as thermophilic bacteria,which is an ideal property for industrial applications. By molecular biological means,TM1525 was cloned into p HT43 vector and introduced into Bacillus subtilis( B. subtilis) WB800 N by electroporation. The results showed that the WB800 N expression system was successfully constructed, and extracellular expression of the recombinant gene was achieved. Cellulose hydrolyzed activity of the protein was exhibited.

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基金项目(Foundation): National Natural Science Foundation of China(Regional Fund)(No.51863020)

作者(Author): 郝亚荣;陈婷;张兴群;

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